Frozen Banks

With a successful expedition in November of 2017 to Heron Island on the Great Barrier Reef, our frozen banks now include sperm from four more coral species (bolded below), for a total of 21.

 

We also expanded our collection of genetic material from three species that were already represented in our banks.  This kind of conservation work is not just a question of freezing reproductive cells from each species; genetic diversity within a species is equally important.  Without a diverse genome, species are far less able to evolve and survive in difficult conditions.  This effect is called a genetic bottleneck.

 

All species in our banks are listed below with links to the World Register of Marine Species (WoRMS), the Ocean Biogeographic Information System (OBIS), and Wikipedia, if an article exists.

 

Great Barrier Reef, Australia

 

Hawaii

  • Fungia scutaria (mushroom coral, a.k.a. Lobactis scutaria) [WoRMS] [OBIS] [Wiki]
  • Montipora capitata (rice coral) [WoRMS] [OBIS] [Wiki]
  • Pocillopora meandrina (cauliflower coral) [WoRMS] [OBIS] [Wiki]

 

Puerto Rico

 

Belize

 

Key Largo, Florida

 

Read on to find out more about our frozen banks.

 

The Reef Recovery Initiative is using human fertility techniques to save endangered and threatened coral species around the world. With protocols similar to those found in human sperm banks, the Reef Recovery team has worked to develop techniques that allow us to freeze and bank coral sperm and stem cells, and in the future, possibly even adult coral fragments. Coral genetic material frozen in this way can be viable for hundreds of years. The frozen sperm can be used to generate new corals and to strengthen small populations by adding genetic diversity. Advances in human stem cell biology may one day allow the frozen stem cells to produce new adults.

In 2010 the first frozen Hawaiian Coral repository was created with sperm and stem cells from two species of corals from Kaneohe Bay.


Interns Kelly Martorana and Malia Peresa help to create Hawaii’s first frozen coral repository. Photo by V. Carter.

Techniques developed working on healthy corals in Hawaii have been taken out into the field from Puerto Rico to Singapore and applied to more impacted corals around the world.


Coral sperm samples ready for freezing in our field-ready cryo-box. Photo by V. Carter.

Whenever the Reef Recovery team freezes sperm from a new coral species, sub-samples of that frozen sperm are thawed and tested on fresh coral eggs to ensure that the sperm will be viable when needed in the future.


Virginia Carter and Jason Acker assess fertilization rates of cryopreserved sperm from the endangered species Acropora palmata or elkhorn coral. Photo by M. Laterveer-Beer.

Our work with SECORE in Puerto Rico has resulted in the banking of the endangered species Acropora palmata or elkhorn coral. In August 2011, we will travel to Belize to bank another endangered species, Acropora cervicornis or staghorn coral. In November 2011, we traveled to Australia to begin training scientists there to start an Australian coral bank.


A dry shipper awaits samples of the endangered elkhorn coral at our field site in Puerto Rico to be shipped to banks at the USDA National Animal Germplasm Program and Omaha’s Henry Doorly Zoo. Photo by M. Laterveer-Beer.

The Reef Recovery team is in the initial stages of research to freeze adult coral polyps as well. This work is preliminary but promising. Someday frozen coral banks may contain whole adult corals with no need for fertilization from gametes or differentiation from stem cells.


Adult coral fragments are inverted and immersed in a solution of cryoprotectant to test the toxicity of the solution to the coral. Photo by V. Carter.

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